Table of Contents
ISRN Veterinary Science
Volume 2013 (2013), Article ID 593029, 5 pages
http://dx.doi.org/10.1155/2013/593029
Research Article

Isolation and Characterization of Mycoplasma mycoides Subspecies capri from Milk of Natural Goat Mastitis Cases

1Central Institute for Research on Goats, Makhdoom, P.O. Farah, Mathura 281122, Uttar Pradesh, India
2Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, Uttar Pradesh, India

Received 4 April 2013; Accepted 24 April 2013

Academic Editors: P. Butaye, J. Cabaret, and I. Nsahlai

Copyright © 2013 Vijay Kumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Association of Mycoplasma mycoides subspecies capri (Mmc) with natural goat mastitis has been studied earlier largely by detecting the Mmc DNA using molecular methods. However, report on detection of cultivable Mmc isolates from natural goat-mastitis milk is still very rare. In this study, Mmc was isolated from milk samples ( ) of goats with or without clinical signs of mastitis. Mmc isolates were further characterized by biochemical and species-specific PCR methods. Intra species strain variation was also studied by 16S amplified rDNA restriction analysis (16S ARDRA). The study recovered a total of 6 Mmc isolates (3.5%). Three types of intraspecies variants among the recovered Mmc isolates were found by 16S ARDRA. The study concluded that Mmc may be an etiological agent of mycoplasmal mastitis in Indian goat herds.