International Scholarly Research Notices / 2013 / Article / Tab 1 / Research Article
Isolation and Characterization of Mycoplasma mycoides Subspecies capri from Milk of Natural Goat Mastitis Cases Table 1 Details of primers used in study.
Primer Specificity to species Sequence Target gene/region Annealing temperature Reference P4/P6 Mmc
* PCR5′-ACTGAGCAATTCCTCTT-3′ 5′-TTAATAAGTCTCTATATGAAT-3′ CAP-21 46°C, 90 sec [16 ] P4/P5 Mmm LC*5′-ACTGAGCAATTCCTCTT-3′ 5′-TTAAATAAGTTTGTATATGAAT-3′ CAP-21 54°C, 30 sec [16 ] Mag-F/Mag-R M . ag *5′-CCTTTTAGATTGGGATAGCGGATG-3′ 5′-CCGTCAAGGTAGCGTCATTTCCTAC-3′ 16S rRNA 60°C, 60 sec [17 ] MputF/MputR M . put *5′-AAATTGTTGAAAAATTAGCGCGAC-3′ 5′-CATATCATCAACTAGATTAATAGTAGCACC-3′ Arc B 52°C, 15 sec [18 ] MCCPL1-L/MCCPL1-R Mcc
*
5′-AGACCCAAATAAGCCATCCA-3′ 5′-CTTTCACCGCTTGTTGAATG-3′ LppA 51°C [19 ] P67BG7-L/P67BG7-R Mbg 7*5′-GGTAATTCGAATAATGATCCT-3′ 5′-TAAGTTTATTGAATTAAAGCG-3′ P67 gene 46°C [20 ]
Mmc : M . mycoides subsp. capri , Mmm LC: M . mycoides susp. mycoides large colony type, M . ag : M . agalactiae , Mcc : M . capricolum subsp. capricolum , Mbg7 : M . bovine group 7.