Competitive HIF Prolyl Hydroxylase Inhibitors Show Protection against Oxidative Stress by a Mechanism Partially Dependent on Glycolysis
Figure 1
Cellomics images showing the algorithm used for quantification of nuclear and cytoplasmic levels of HIF-1α. Raw images without the applied algorithm used to define cells are shown in the top panel and images showing the algorithm are shown in the lower panel. Circ is defined by the outline of the Hoechst-staining and thus represents the nuclear region. Ring is defined as a certain radius surrounding the Circ region and thus represents the cytoplasmic region of the cell. The Hoechst fluorescence and the HIF-1α fluorescence are recorded in two different channels.
