Competitive HIF Prolyl Hydroxylase Inhibitors Show Protection against Oxidative Stress by a Mechanism Partially Dependent on Glycolysis
Figure 5
Cpd A is partially protective even when glycolysis is inhibited. (a) ATP-levels in SH-SY5Y cells grown overnight in starvation media overnight, then treated with 10 mM 2-deoxyglucose for 3 hours (or nothing for control) followed by 3 hours of treatment with 6-OHDA at the indicated concentrations. (b) and (c) ATP-levels in SH-SY5Y cells grown over night in starvation media, then pretreated for 3 hours with 10 mM 2-deoxyglucose (or nothing for control) and Cpd A (25 or 50 μM or nothing for control) followed by 3 hours treatment with 100 μM 6-OHDA (b) or 300 μM 6-OHDA (c).
