Research Article

Protective Effects of Guava Pulp on Cholestatic Liver Injury

Figure 2

Effect of guava pulp on hepatobiliary system and biliary epithelial cell proliferation. (a) The hepatobiliary system at days 1, 7, and 28. Progressive liver injury appeared at LMBDL groups from days 1 to 28. Representative H&E (×200) results of the ligated livers from the LMBDL group (b), the ligated livers from the LMBDL+GP group (c), the unligated livers from the LMBDL (d), and the unligated livers from LMBDL+GP (e), respectively. Arrow (↑)  =  biliary epithelial cell proliferation. (f) Representative Ki-67 immunohistochemical results of the proliferative ductules in the LMBDL group at day 28. Arrow  =  Ki-67 positive biliary epithelial cells. ((g)-(h)) Quantification of Ki-67 positive biliary epithelial cells and bile duct mass. Ki-67 and CK-19 results of biliary epithelial cells in the left and median lobes were evaluated for proliferation. , the liver tissues from the sham operation versus the ligated lobes in LMBDL and LMBDL+GP groups. , the ligated lobes of LMBDL at day 1 versus the ligated lobes at days 7 and 28.