Protective Effects of Guava Pulp on Cholestatic Liver Injury
Figure 4
Effects of guava pulp on liver fibrogenesis, relative gene expression. (a) Representative pictures of liver fibrogenesis at days 1, 7, and 28 following LMBDL (top row) and LMBDL+GP (bottom row) by Sirius Red stain (200x). (b) Percentage of liver surface stained with Sirius red. Values were mean (s.e.m.) for five to six individual animals per time point. , the ligated lobes of LMBDL versus LMBDL+GP at days 1, 7, and 28, respectively. ((c)–(e)) Levels of procollagen 1α1, metalloproteinases 1 and transforming growth factor mRNA are determined by reverse transcriptase-polymerase chain reaction and expressed as fold induction in comparison to sham-operated controls. Values were mean (s.e.m.) for five to six individual animals per time point. (c) Expression of procollagen 1α1. , the ligated lobes of LMBDL+GP groups at days 1, 7, and 28 versus the ligated lobes of LMBDL groups at days 1, 7, and 28, respectively. (d) Expression of metalloproteinases 1. the ligated lobes of LMBDL groups at days 1, 7, and 28 versus the ligated lobes of LMBDL+GP groups at days 1, 7, and 28, respectively. (e) Expression of TGF-β1. , the ligated lobes of LMBDL at days 1, 7, and 28 versus the ligated lobes of LMBDL+GP at days 1, 7, and 28, respectively.
