DLK1 Protein Expression during Mouse Development Provides New Insights into Its Function

Figure 1

Expression of DLK1 protein in pre- and postnatal liver. (a) It shows a western blot of embryonic liver lysates incubated with DLK1-A17 antibody. DLK1 protein expression in the liver increases up to ED16 and declines thereafter. The most pronounced DLK1 variant is the 50 kDa band (see (b) for a comparison of long and short exposures). Particularly at ED14 and 15, a pronounced 55 kDa band (right panel in (b)) is also present. This 55 kDa band was the only expressed DLK1 protein variant in confluent 3T3L1 cells. The 50 and 55 kDa bands represent the transmembrane and cleaved variants of the DLK1 protein, respectively. (a′) It shows protein loading per lane, visualized with Amidoblack.