Table of Contents
ISRN Biomaterials
Volume 2013, Article ID 629807, 6 pages
http://dx.doi.org/10.5402/2013/629807
Research Article

Chitosan-Carrageenan Polyelectrolyte Complex for the Delivery of Protein Drugs

1School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459
2School of Life Sciences & Chemical Technology, Ngee Ann Polytechnic, Singapore 599489
3Department of Chemical Engineering, The Petroleum Institute, P. O. Box 2533, Abu Dhabi, UAE

Received 22 July 2012; Accepted 12 August 2012

Academic Editors: S. Lamponi and M. Rouabhia

Copyright © 2013 Cunben Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A chitosan-carrageenan polyelectrolyte complex (PEC) was prepared by salt induced impeding of polyplex formation method and was encapsulated with bovine serum albumin (BSA) to study the potential to be tailored to the pH responsive oral delivery of protein drugs. The FTIR spectra showed the successful formation of the PEC under the experimental condition. The release kinetics of BSA from the PEC was studied in the simulated gastrointestinal fluids with and without digestive enzymes. The prepared PEC showed the nature of pH-sensitivity. A typical controlled release of BSA from the PEC (180 μg of BSA from 3 mg of PEC) was obtained in the simulated intestinal fluid (SIF, pH 7.5), which was due to the significant swelling and disintegration of PEC, but little amount of BSA was released (11 μg of BSA from 3 mg of PEC) in the simulated gastric fluid (SGF, pH 1.2), confirming acidic stability of the prepared PEC. The presence of digestive enzymes was found not to affect the response of PEC to ambient pH value, but to speed up the release of BSA from carriers.