SUMOhunt: Combining Spatial Staging between Lysine and SUMO with Random Forests to Predict SUMOylation

Figure 1

The SUMOylation pathway: the SUMO pathway is categorized into maturation, activation, conjugation, and ligation. First of all, SENPs convert immature SUMO to mature SUMO by exposing diglycine motif at carboxyl terminal. This mature SUMO is activated by AOS1/UBA2 (E1—activating enzyme) with the help of ATP-dependent reaction. Activated SUMO conjugates with E2—conjugating enzyme ubc9—which finally transfers it to target lysine on substrate protein with the help of E3 ligase.