Table of Contents
ISRN Environmental Chemistry
Volume 2013 (2013), Article ID 684297, 7 pages
http://dx.doi.org/10.1155/2013/684297
Research Article

Closed Anaerobic Biotransformation Products of Organoarsenic Compounds in Fucus distichus

Department of Chemical Sciences, College of Sciences, Afe Babalola University, Ekiti State, Ado-Ekiti 360211, Nigeria

Received 27 April 2013; Accepted 11 June 2013

Academic Editors: N. Belzile, A. Waseem, and C. Waterlot

Copyright © 2013 Abiodun A. Ojo and Amos Onasanya. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The closed anaerobic decomposition extracts of Fucus distichus incubated with seawater and sediment, and without sediment as control, were subjected to extractions and isolation on Sephadex LH 20 and Cellulose Thin Layer Chromatography. The decomposition extracts and isolates were analyzed by using both the Hydride Generation Gas Chromatography Atomic Absorption Spectrometry (HG-GC-AAS) and High Performance Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry (HPLC-ICPMS) to identify the arsenic species in the equilibrium mixtures of the seaweed and filtrates separately. In the methanol seaweed extract, equilibrium mixture of arsenosugars (AS) AS1 and AS2 and their biotransformation products of dimethylarsinoylethanol (DMAE) and dimethylarsinic acid (DMAA) were identified. In the methanol filtrate extract of the mixture, only DMAE and DMAA were identified. However, in the control methanol filtrate extract five organoarsenic species, AS1 and AS2, one unidentified hidden organoarsenic species, DMAE and DMAA were identified in the equilibrium mixture. This result confirmed that the hidden organoarsenic species in Fucus distichus, AS1 and AS2, and an unidentified organoarsenic compounds are biotransformed to only DMAE and DMAA under an anaerobic condition. This also suggests that DMAE and DMAA are strong intermediate candidates for the generation of arsenobetaine, from arsenoribosides in the marine food webs.