MHC Disparate Resting B Cells Are Tolerogenic in the Absence of Alloantigen-Expressing Dendritic Cells

Figure 4

Rejection of D^d skin grafts in adoptively transferred mice. T cells (a) or purified CD8⁺ T cells (b) from D^d primed or naive FVBs were transferred into ATX FVB mice 1 week after irradiation (500 cGy). Mice were infused with CD2-D^d B cells 1 wk after T cell transfer, and were grafted with MHC-D^d tail skin 2 wks later. MST of skin grafts: naive CD8⁺ T cell-infused mice (open square) = 18.5 days (𝑛=4); naive CD8⁺ T cell plus CD2-D^d B cell-treated mice (filled square) = 35.5 days (𝑛=4); primed CD8⁺ T cell plus CD2-D^d B cell-treated mice (filled circle) = 17.5 days (𝑛=4); primed CD8⁺ T cell-infused mice (open circle) = 7.5 days (𝑛=4); irradiation alone (x) = 32.5 days (𝑛=4).

(a)

(b)