Table of Contents
ISRN Radiology
Volume 2013 (2013), Article ID 704659, 7 pages
http://dx.doi.org/10.5402/2013/704659
Clinical Study

Investigating γ H2AX as a Biomarker of Radiosensitivity Using Flow Cytometry Methods

1Environmental and Radiation Health Sciences Directorate, Health Canada, 775 Brookfield Road, Postal Locator 6303B, Ottawa, ON, Canada K1A 0K9
2Department of Physics, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada
3The Ottawa Hospital Cancer Centre, 501 Smyth Road, Ottawa, ON, Canada

Received 18 January 2013; Accepted 19 March 2013

Academic Editors: M. G. Andreassi and A. Sciarra

Copyright © 2013 Lindsay A. Beaton et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. B. Emami, J. Lyman, A. Brown et al., “Tolerance of normal tissue to therapeutic irradiation,” International Journal of Radiation Oncology, Biology, Physics, vol. 21, no. 1, pp. 109–122, 1991. View at Google Scholar · View at Scopus
  2. S. M. Bentzen and J. Overgaard, “Patient-to-patient variability in the expression of radiation-induced normal tissue injury,” Seminars in Radiation Oncology, vol. 4, no. 2, pp. 68–80, 1994. View at Google Scholar · View at Scopus
  3. L. A. Henriquez-Hernandez, E. Bordon, B. Pinar, M. Lloret, C. Rodriguez-Gallego, and P. C. Lara, “Prediction of normal tissue toxicity as part of the individualized treatment with radiotherapy in oncology patients,” Surgical Oncology, vol. 21, pp. 201–206, 2012. View at Google Scholar
  4. O. A. Sedelnikova, D. R. Pilch, C. Redon, and W. M. Bonner, “Histone H2AX in DNA damage and repair,” Cancer Biology and Therapy, vol. 2, no. 3, pp. 233–235, 2003. View at Google Scholar
  5. E. P. Rogakou, C. Boon, C. Redon, and W. M. Bonner, “Megabase chromatin domains involved in DNA double-strand breaks in vivo,” Journal of Cell Biology, vol. 146, no. 5, pp. 905–915, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. P. L. Olive and J. P. Banáth, “Phosphorylation of histone H2AX as a measure of radiosensitivity,” International Journal of Radiation Oncology, Biology, Physics, vol. 58, no. 2, pp. 331–335, 2004. View at Publisher · View at Google Scholar · View at Scopus
  7. C. E. Rübe, A. Fricke, J. Wendorf et al., “Accumulation of DNA double-strand breaks in normal tissues after fractionated irradiation,” International Journal of Radiation Oncology, Biology, Physics, vol. 76, no. 4, pp. 1206–1213, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. A. Menegakis, A. Yaromina, W. Eicheler et al., “Prediction of clonogenic cell survival curves based on the number of residual DNA double strand breaks measured by γH2AX staining,” International Journal of Radiation Biology, vol. 85, no. 11, pp. 1032–1041, 2009. View at Google Scholar
  9. J. Werbrouck, K. De Ruyck, L. Beels et al., “Prediction of late normal tissue complications in RT treated gynaecological cancer patients: potential of the γ-H2AX foci assay and association with chromosomal radiosensitivity,” Oncology Reports, vol. 23, no. 2, pp. 571–578, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. M. L. K. Chua, N. Somaiah, R. A'Hern et al., “Residual DNA and chromosomal damage in ex vivo irradiated blood lymphocytes correlated with late normal tissue response to breast radiotherapy,” Radiotherapy and Oncology, vol. 99, no. 3, pp. 362–366, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. A. Andrievski and R. C. Wilkins, “The response of γ-H2AX in human lymphocytes and lymphocytes subsets measured in whole blood cultures,” International Journal of Radiation Biology, vol. 85, no. 4, pp. 369–376, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. L. A. Beaton, C. Ferrarotto, L. Marro et al., “Chromosome damage and cell proliferation rates in in vitro irradiated whole blood as markers of late radiation toxicity after radiation therapy to the prostate,” International Journal of Radiation Oncology, Biology, Physics, vol. 85, no. 5, pp. 1346–1352, 2013. View at Google Scholar
  13. J. D. Cox, J. Stetz, and T. F. Pajak, “Toxicity criteria of the Radiation Therapy Oncology Group (RTOG) and the European Organization for Research and Treatment of Cancer (EORTC),” International Journal of Radiation Oncology, Biology, Physics, vol. 31, no. 5, pp. 1341–1346, 1995. View at Publisher · View at Google Scholar · View at Scopus
  14. S. Chow, D. Hedley, P. Grom, R. Magari, J. W. Jacobberger, and T. V. Shankey, “Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations,” Cytometry Part A, vol. 67, no. 1, pp. 4–17, 2005. View at Publisher · View at Google Scholar · View at Scopus
  15. M. Lew, “Good statistical practice in pharmacology Problem 2,” British Journal of Pharmacology, vol. 152, no. 3, pp. 299–303, 2007. View at Publisher · View at Google Scholar · View at Scopus