Investigating γ H2AX as a Biomarker of Radiosensitivity Using Flow Cytometry Methods

Table 2

Comparing control to sensitive groups for the dose course experiments. Cells in table represent the geometric mean (and standard deviation) of the fluorescent intensity of the γH2AX signal for each status and dose combination.

Treatment group	Dose (Gy)
	0	2	4	6	8	10
	CD4^a
Control (n = 20)	8.83 (1.31)	30.73 (6.93)	53.91 (10.44)	75.92 (17.80)	101.98 (26.90)	117.81 (31.73)
Sensitive (n = 10)	8.83 (2.58)	32.53 (7.97)	51.37 (11.41)	75.33 (17.44)	98.44 (19.83)	114.41 (30.85)
	CD8^b
Control (n = 20)	8.25 (1.50)	24.82 (5.86)	42.00 (8.89)	57.09 (14.41)	76.31 (24.05)	86.12 (24.94)
Sensitive (n = 10)	7.84 (1.84)	25.22 (5.16)	38.79 (6.13)	53.57 (11.21)	70.45 (16.58)	82.55 (19.24)
	CD19^c
Control (n = 20)	7.61 (1.59)	19.99 (4.81)	33.81 (7.90)	45.66 (11.69)	59.37 (18.40)	66.38 (20.61)
Sensitive (n = 10)	6.97 (2.15)	21.07 (5.73)	31.82 (6.39)	42.91 (9.79)	55.31 (10.43)	62.32 (15.43)
	Lymphocytes^d
Control (n = 20)	8.39 (1.29)	26.49 (6.17)	45.08 (9.49)	61.32 (15.44)	81.72 (24.08)	92.39 (26.90)
Sensitive (n = 10)	8.32 (2.20)	27.61 (6.32)	42.10 (7.44)	58.83 (11.93)	75.97 (14.23)	87.49 (21.26)

Overall status effect: ^a𝐹_(1,28) (P value) = 0.06 (0.81), ^b𝐹_(1,28) (P value) = 0.38 (0.54), ^c𝐹_(1,28) (P value) = 0.33 (0.57), ), and ^d𝐹_(1,28) (P value) = 0.29 (0.59).
Overall difference between status groups at various dose groups: ^a𝐹_(5,140) (P value) = 0.18 (0.97), ^b𝐹_(5,140) (P value) = 0.33 (0.89), ^c𝐹_(5,140) (P value) = 0.42 (0.84), and ^d𝐹_(5,140) (P value) = 0.42 (0.84).