Investigating γ H2AX as a Biomarker of Radiosensitivity Using Flow Cytometry Methods
Table 3
Comparing control to sensitive groups for time course experiments after 2 Gy. Cells in table represent the geometric mean (and standard deviation) of the fluorescent intensity of the γH2AX signal for each status and time combination.
Treatment group
Time (hrs)
0
0.5
1
2
4
8
24
CD4a
Control (n = 20)
11.01 (3.50)
33.38 (8.43)
40.40 (11.07)
35.40 (8.10)
24.42 (6.33)
13.23 (2.93)
10.30 (2.03)
Sensitive (n = 10)
10.80 (3.86)
32.18 (7.81)
40.41 (8.39)
37.91 (11.26)
25.87 (7.81)
14.25 (4.07)
10.26 (3.14)
CD8b
Control (n = 20)
10.09 (3.32)
29.02 (7.79)
34.44 (9.66)
29.87 (7.26)
21.31 (5.94)
12.36 (3.02)
9.72 (2.17)
Sensitive (n = 10)
9.13 (2.64)
27.27 (5.22)
33.18 (5.03)
30.29 (6.51)
21.26 (5.65)
12.70 (3.03)
9.37 (2.51)
CD19c
Control (n = 20)
11.38 (5.29)
25.14 (8.47)
29.45 (9.35)
26.54 (7.42)
19.30 (6.22)
11.64 (3.86)
10.26 (2.98)
Sensitive (n = 10)
9.85 (4.90)
24.18 (6.56)
28.48 (6.24)
26.25 (8.69)
18.54 (6.25)
11.56 (3.32)
9.69 (3.21)
Lymphocytesd
Control (n = 20)
10.54 (3.13)
30.50 (7.50)
36.92 (9.79)
32.54 (7.41)
22.82 (5.77)
13.22 (3.02)
10.72 (2.20)
Sensitive (n = 10)
10.28 (3.07)
29.27 (5.80)
36.59 (5.84)
33.99 (7.99)
23.65 (6.37)
14.03 (3.47)
10.72 (3.05)
Overall status effect: a (P value) = 0.05 (0.82),b (P value) = 0.11 (0.74), c (P value) = 0.15 (0.70), and d (P value) = 0.01 (0.94). Overall difference between status groups at the various time points: a (P value) = 0.38 (0.89), b (P value) = 0.25 (0.96),c (P value) = 0.08 (1.00), and d (P value) = 0.27 (0.95).