A C-Terminal Transmembrane Anchor Targets the Nuage-Localized Spermatogenic Protein Gasz to the Mitochondrial Surface
Figure 4
Gasz can recruit piRNA-processing proteins to the mitochondria. HA- and FLAG-tagged expression constructs for RanBP9, Mili, and MVH were transfected or cotransfected with EGFP-Gasz into Hela cells and processed for imaging as before. (c, e) N, nucleus; asterisk, non-Mili- or MVH-cotransfected cells; arrowheads, cotransfected cells. (d) Arrow, endoplasmic reticulum-like structure. Images are ones typical of at least 3 experiments.