Table of Contents
ISRN Microbiology
Volume 2013, Article ID 728134, 7 pages
http://dx.doi.org/10.1155/2013/728134
Research Article

Isolation, Identification, and Characterization of a Cellulolytic Bacillus amyloliquefaciens Strain SS35 from Rhinoceros Dung

1Center for Energy, Indian Institute of Technology Guwahati, Guwahati, Assam 781 039, India
2Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781 039, India
3Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781 039, India

Received 30 March 2013; Accepted 8 May 2013

Academic Editors: L. Brusetti, J. Kreth, M. C. Lai, and J. Maupin-Furlow

Copyright © 2013 Shuchi Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cellulose hydrolyzing bacteria were isolated from rhinoceros dung and tested for clear zone formation around the colonies on the agar plates containing the medium amended with carboxymethylcellulose as a sole carbon source. Isolates were further screened on the basis of carboxymethylcellulase production in liquid medium. Out of 36 isolates, isolate no. 35 exhibited maximum enzyme activity of 0.079 U/mL and was selected for further identification by using conventional biochemical tests and phylogenetic analyses. This was a Gram-positive, spore forming bacterium with rod-shaped cells. The isolate was identified as Bacillus amyloliquefaciens SS35 based on nucleotide homology and phylogenetic analysis using 16S rDNA and gyrase A gene sequences.