Differentiation of Human Umbilical Cord Lining Membrane-Derived Mesenchymal Stem Cells into Hepatocyte-Like Cells
Figure 3
RT-PCR analysis of gene expression. In the hepatogenic medium, cells expressed ALB, AFP, CK18, and CK19 after 21 days (a). (Lane 1: noninduced MSCs-negative control, lane 2: differentiated cells; lane 3: HepG2-positive control.) In the osteogenic medium, induced cells expressed osteopontin and osteocalcin after 14 days (lane 1: 0 day, lane 2: 7 days; lane 3: 14 days) (b). GAPDH serves as an internal control.
