Differentiation of Human Umbilical Cord Lining Membrane-Derived Mesenchymal Stem Cells into Hepatocyte-Like Cells
Figure 5
Real-time qRT-PCR analysis of hepatocyte-specific gene expression and Western blot analysis of hepatocyte-specific marker expression. Total RNA was extracted from CLMSC on days 0, 7, 14, and 21 following hepatocyte induction. The mRNA expression of hepatocyte-specific genes, such as ALB, AFP, CK19, and CK18 was analyzed by real-time RT-PCR and normalized with GAPDH gene. relative to day 0 () (a). Total cellular protein was extracted from CLMSCs on days 0, 7, 14, and 21 following hepatocyte induction. After electrophoresis and electrotransfer to membranes, the membranes were incubated with mouse Ab against the hepatocyte-specific markers ALB, AFP, CK19 and CK18 and then with HRP-linked goat anti-mouse IgG. β-Actin was used as a housekeeping gene control. The size of each protein is indicated (b).
