Effect of Ghrelin on Hepatic IGF-Binding Protein-1 Production

Figure 2

Ghrelin enhances the stimulatory effect of AICAR on IGFBP-1 secretion by H4-II-E cells. Confluent cells were exposed to ghrelin (100 nM) and AICAR (200 μM) in the absence and presence (hatched bars) of 10 ng/mL insulin for a 5 h incubation period under serum-free conditions. IGFBP-1 concentrations, measured by specific immunoassay, pooled from three experiments each containing 4–6 replicates per condition, are expressed as a percentage of the secretion by control wells. *𝑃<0.001 (effect of insulin compared to condition in the absence of insulin); ^†𝑃<0.001 (effect of AICAR compared to control wells); ^‡𝑃<0.001 (compared to AICAR alone); ^§𝑃<0.001 (compared to insulin alone).