Table of Contents
ISRN Tissue Engineering
Volume 2013, Article ID 752620, 7 pages
Research Article

Hyaluronan-Based Three-Dimensional Microenvironment Potently Induces Cardiovascular Progenitor Cell Populations

1Cardiovascular Tissue Engineering Laboratory, Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, 10833 Le Conte Avenue, 62-151 CHS, Los Angeles, CA 90095-1741, USA
2Cardiovascular Research Laboratory, Departments of Medicine and Cardiology, David Geffen School of Medicine, University of California, Los Angeles, 675 Charles E. Young Drive South, MRL 3645, Los Angeles, CA 90095, USA
3Department of Medicine and Cardiology, University of Washington, 850 Republican Street, Brotman Room 432, Seattle, WA 98109, USA

Received 19 June 2013; Accepted 31 July 2013

Academic Editors: C. Haasper and Y.-J. Ren

Copyright © 2013 Jessica M. Gluck et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The relationship between stem cell niches in vivo and their surrounding microenvironment is still relatively unknown. Recent advances have indicated that extrinsic factors within the cardiovascular progenitor cell niche influence maintenance of a multipotent state as well as drive cell-fate decisions. We have previously shown the direct effects of extracellular matrix (ECM) proteins and have now investigated the effects of dimension on the induction of a cardiovascular progenitor cell (CPC) population. We have shown here that the three-dimensionality of a hyaluronan-based hydrogel greatly induces a CPC population, as marked by Flk-1. We have compared the effects of a 3D microenvironment to those of conventional 2D cell culture practices and have found that the 3D microenvironment potently induces a progenitor cell state.