Figure 2: Percentages of Treg cells in spleen (a) and blood (b) of C3 tumor-challenged mice at week five after challenge. Groups of tumor-implanted mice were injected with PBS alone, or vaccinated using DPX-E7 vaccine, or injected as CE vaccine emulsion. Percentages of CD4+CD25+Foxp3+ Treg cells in spleen and blood were determined using flow cytometry. Data represents mean ± SDM from at least 5 mice per group and from one of three experiments with similar findings.