Figure 6: Retention behavior of Hg2+, CH3Hg+, and Cd2+ on a Gemini C18 RP-HPLC column (150 × 4.6 mm I.D.) at 37°C. Mobile phase: 5.0 mM GSH with 0–10 mM Cys in PBS buffer (pH 7.4). Flow rate: 1.0 mL/min. Detector: ICP-AES (Hg at 194.227 nm or Cd at 226.502 nm). Injection volume: 20 μL (5 μg Hg or Cd). Detached symbols on right side correspond to the of each metal species using PBS buffer with 5.0 mM Cys as the mobile phase. Errors in are within the area of plot markers unless otherwise indicated (reproduced with permission from [121] and Elsevier).