Estrogen Signaling and the Aging Brain: Context-Dependent Considerations for Postmenopausal Hormone Therapy

Table 1

Protein interactions with ER𝛽 were altered by age and 𝐸₂. Selected proteins that were significantly altered (𝑃<0.05) in their association with ER𝛽 depending on age and 𝐸₂ treatment. Experimental paradigm: young (3 month) and aged (18 month) female Fischer 344 rats were ovariectomized and hormone deprived for 7 days. Following deprivation, animals were administered 2.5 𝜇g/kg 𝐸₂ (plasma levels = 79.45 ± 22.5 pg/mL) or vehicle (safflower oil) via subcutaneous injection once/day for 3 days. Nuclear protein was isolated from the ventral hippocampus and coimmunoprecipitated for ER𝛽 (a beam 14C8) and associated proteins. Protein interactions were identified and quantified using 2D-DIGE/DeCyder and ESI MS/MS. YV = young + vehicle; YE = young + 𝐸₂; AV = aged + vehicle; AE = aged + 𝐸₂.

Accession no.	Molecular; weight (Kda)	Estimated; isoelectric; point	PEAKS; score	% Coverage	ID	Interaction with ER𝛽				Function
						Young; vehicle	Young; 𝐸2	Aged; vehicle	Aged; 𝐸2
gi∣ 149038929	80	5.75	49.4	6.43	Gelsolin	—	↑	—	—	Actin-binding coactivator
gi∣ 116242507	75	5.97	93	14.58	Heat-shock protein 70	—	↓	—	↑	Chaperone
gi∣ 120538378	47	5.7	93.2	10.72	Heterogeneous nuclear ribonucleoprotein H1/2	—	↑	—	—	RNA splicing