Table of Contents
ISRN Toxicology
Volume 2013 (2013), Article ID 817353, 6 pages
http://dx.doi.org/10.1155/2013/817353
Research Article

Genotoxicological Evaluation of NUTRALYS Pea Protein Isolate

1Biology and Nutrition Department, Roquette Frères, 62136 Lestrem, France
2International Institute of Biotechnology and Toxicology (IIBAT), Padappai, Tamil Nadu 601301, India

Received 21 December 2012; Accepted 27 January 2013

Academic Editors: S. T. Chu, A. Hakura, S. V. Kala, M. Valverde, S. M. Waliszewski, and K. Wohlfarth

Copyright © 2013 Chentouf Aouatif et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

NUTRALYS Pea Protein Isolate, a protein supplement, is a high-quality source of protein which is primarily emulsifying functional protein. We evaluated the genotoxic potential of NUTRALYS isolated from dry yellow pea, using three established genotoxicity tests (AMES test in vitro chromosomal aberration test, and in vivo micronucleus test) employing OECD guidelines under GLP conditions. In the bacterial reverse mutation test, NUTRALYS did not show positive responses in strains detecting point and frame shift mutations. In the chromosomal aberration test, NUTRALYS did not induce chromosome aberrations in the presence and absence of metabolic activation. In the bone marrow micronucleus test, NUTRALYS did not induce significant increases of micronucleated immature (polychromatic) erythrocytes in bone marrow of test animals.