Nanocomposites Based on PLLA and Multi Walled Carbon Nanotubes Support the Myogenic Differentiation of Murine Myoblast Cell Line
Figure 2
Interaction of C2C12 with PLLA and PLLA/MWCNTs nanocomposite films. Murine C2C12 myoblasts were cultured on PLLA, 0.1 MWCNTs, 0.5 MWCNTs, 1 MWCNTs, and 3 MWCNTs. (a) XTT viability assay of C2C12 plated on different substrates, at 3, 7, and 14 days. Results were comparable for cells grown on smoothness (●) and roughness (○) surfaces. (b) Representative images of C2C12 seeded on each substrate, at 3, 7, and 14 days. Microtubule organization is showed by -tubulin (tetramethylrhodamine isothiocyanate (TRIC))/F-actin (fluorescein isothiocyanate (FITC))/,6-diamidino-2-phenylindole (DAPI) staining. Images were captured with 20x objectives; scale bar: 100 m. (c) Elongation factor (11) was measured for cells cultured on each substrate, on smoothness (■) and roughness (□) surfaces, at different intervals of time (1, 3, 7, and 14 days). Each experiment consisted of at least five technical replicates. Values are expressed as means SEM .
