Nanocomposites Based on PLLA and Multi Walled Carbon Nanotubes Support the Myogenic Differentiation of Murine Myoblast Cell Line
Figure 3
MyoD expression in C2C12 cultured on neat PLLA and PLLA/MWCNTs. Immunofluorescences of murine C2C12 cultured in 2% FBS containing medium for 2 days express the early myogenic marker MyoD (RED) either in neat PLLA and PLLA/MWCNTs. Cell nuclei (BLUE) were stained with DAPI. Scale bar: 100 m. Graph reports MyoD expression. Level is defined as the number of MyoD positive nuclei (■) in a given image and was calculated in differentiated cells cultivated on each substrate. As reference numbers of MyoD negative nuclei are reported (□). Each experiment consisted of at least five technical replicates. Values are expressed as means SEM ).
