Nanocomposites Based on PLLA and Multi Walled Carbon Nanotubes Support the Myogenic Differentiation of Murine Myoblast Cell Line
Figure 5
Myogenic parameters in C2C12 after differentiation on neat PLLA and PLLA/MWCNTs. (a) Myogenic index, defined as the number of 2-3 nuclei residing in myotubes divided by total number of nuclei in a given image, was calculated in differentiated cells cultivated on different substrates. Measurement of length (b) and width (c) of myotubes. Quantitative analysis of the morphology of myotubes after 14 days of differentiation was performed by using the fluorescence microscopy Eclipse-TE2000-S Nikon, equipped with the F-View-II FireWire camera and the Soft Imaging System NIS, Nikon. Average width and average length are reported. Each experiment consisted of at least five technical replicates. Values are expressed as means SEM ().
