Table of Contents
ISRN Nanomaterials
Volume 2013, Article ID 843709, 9 pages
Research Article

Synthesis, Characterization, and Applications of Dendrimer-Encapsulated Zero-Valent Ni Nanoparticles as Antimicrobial Agents

1Chemistry Department, Tuskegee University, Tuskegee, AL 36088, USA
2Biology Department, Tuskegee University, Tuskegee, AL 36088, USA
3Center for Advance Materials, Tuskegee University, Tuskegee, AL 36088, USA

Received 1 July 2013; Accepted 27 August 2013

Academic Editors: Y. Barnakov, J. Gu, Z.-M. Liao, and S. Sun

Copyright © 2013 A. Mazumder et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Dendrimers have emerged as one of the most promising, cost-effective synthesizing methodologies in which highly monodispersed metallic nanoparticles can be produced with varied chemical functionalities. In this report, we have investigated the synthesis and application of as-synthesized dendrimer-encapsulated zero-valent nickel “Ni(0)” nanoparticles (NPs), using a fourth generation (G4) NH2-terminated poly(amido)amine (PAMAM) dendrimer as the host template, as potential antimicrobial agents. Apparently, based on ultraviolet visible spectroscopy (UV-vis) and transmission electron microscopy (TEM) analyses, Ni(0) NPs with an average measured size less than 10 nm in diameter were formed within the interior void cavity of the dendrimer structure. X-ray diffraction (XRD) analysis indicates that the NPs exhibited a single-phased, face-centered-cubic (fcc) crystallographic structure. Furthermore, to evaluate the antimicrobial activity of the dendrimer-encapsulated Ni(0) NPs, disk diffusion assay and minimum inhibitory concentration (MIC) examinations, both antimicrobial tests, were conducted. Subsequently, UV-vis analyses, after exposure of the dendrimer-encapsulated Ni(0) NPs to both Gram-negative and Gram-positive bacteria, revealed that the dendrimer-encapsulated particles prevented the growth of bacteria during the culturing stage.