Table of Contents
ISRN Pharmacology
Volume 2013 (2013), Article ID 849412, 10 pages
http://dx.doi.org/10.1155/2013/849412
Research Article

Some Haematological and Biochemical Investigations on Duck Virus Hepatitis following Administration of Glycyrrhizin

1Department of Parasitology and Animal Diseases, National Research Center, El-Behouse Street, Dokki, P.O. Box 12622, Giza, Egypt
2Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt

Received 19 May 2013; Accepted 2 July 2013

Academic Editors: T. M. Palmer, T. W. Stone, and S. Tsuruoka

Copyright © 2013 Faten A. Okda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study aimed to investigate the protective effect of glycyrrhizin (locally isolated and purified from licorice root) against duck hepatitis virus through the assessment of some hematological and biochemical parameters. One hundred and sixty white Pekin ducklings—one day old—were randomly divided into four equal groups. Group (1) was kept as normal control. Group (2) was inoculated I/P with 10 mg glycyrrhizin/kg BW, three times per week for four weeks. Group (3) was inoculated I/M with 0.5 ml of live attenuated DHV vaccine. Group (4) was inoculated with both glycyrrhizin (10 mg/kg BW I/P, three times per week for four weeks) and live attenuated DHV vaccine (0.5 ml, I/M). Then, all groups of treatment were challenged using virulent DHV except for 20 ducklings from the normal control group which were continued to be kept as negative control. The results revealed that duck hepatitis virus (DHV) caused macrocytic hypochromic anemia, leukopenia, hypoproteinemia, hypoalbuminemia, hyperglycemia, hypercholesterolemia, and marked elevation of liver enzymes and renal parameters. In conclusion, glycyrrhizin injected alone or in combination with DHV vaccine protected or ameliorated the deteriorating effects induced by DHV vaccine and/or duck hepatitis virus infection by improvement of erythrogram and leukogram, as well as liver and kidney functions.