Some Haematological and Biochemical Investigations on Duck Virus Hepatitis following Administration of Glycyrrhizin

Table 3

Leukogram of different experimental groups of ducklings before (at the 1st, 2nd, and the 3rd weeks) and after challenge (at the 4th week) (mean ± standard deviation).

		Groups
Parameters	Periods (weeks)	Control (1)		(2)	(3)	(4)	LSD
		Normal	After challenge	Glycyrrhizin	DHV vaccine	Glycyrrhizin + DHV vaccine
Total leukocytic count (10^3/µL)	1	26.76±0.26		36.80^∗±0.64	21.80^∗±0.30	34.00^∗±0.70	1.70
	2	30.56±1.04		37.00^∗±0.44	29.24±1.22	35.28^∗±0.41	1.60
	3	34.44±1.08		40.12^∗±1.50	34.56±1.44	37.20^∗±1.48	1.86
	4^#	33.80^b±4.08	22.00^∗±0.1	29.80^∗b±0.64	22.80^∗±0.30	28.88^∗b±0.70	3.69
Lymphocyte count (×10^3/µL)	1	20.29±2.5		27.24^∗±2.02	19.04±4.55	28.06^∗±4.34	4.74
	2	24.72±3.72		31.70^∗±2.70	25.93±3.39	35.75^∗±6.18	5.64
	3	24.91±4.16		36.61^∗±2.62	30.68^∗±2.27	36.92^∗±2.54	4.01
	4^#	17.00^b±2.27	19.02^∗±1.00	25.24^∗b±1.02	25.80^∗b±4.55	24.06^∗b±4.34	1.89
Heterophil count; (×10^3/µL)	1	4.34±0.33		4.57±0.19	0.00^∗±0.41	4.20±1.88	1.32
	2	4.30±0.62		2.00±0.00	0.00^∗±0.1	2.46±1.88	0.42
	3	8.04±1.02		0.21±0.00	0.35^∗±1.02	0.00±0.51	0.78
	4^#	10.70^b±0.72	0.00^∗±0.00	1.27^∗b±0.00	0.40^∗±0.70	2.00^∗b±0.00	0.49
Monocyte count (×10^3/µL)	1	0.94±0.00		1.02±0.00	2.76^∗±0.28	1.45^∗±0.02	0.19
	2	1.01±0.25		3.09^∗±0.00	2.36^∗±0.15	1.93^∗±0.06	0.20
	3	1.47±0.00		3.07^∗±0.00	2.45^∗±0.00	1.64^∗±0.00	0.10
	4^#	1.00^b±0.00	2.81^∗±0.98	2.46^∗b±0.00	3.77^∗b±0.00	2.03^∗b±0.00	0.10
Eosinophil count; (×10^3/µL)	1	1.13±0.03		1.00±0.00	0.00^∗±0.00	0.25±0.15	0.10
	2	0.32±0.18		0.21±0.00	0.00^∗±0.00	0.00±0.00	0.12
	3	0.52±0.00		0.08±0.10	0.38^∗±0.01	0.00±0.00	0.07
	4^#	6.20^b±0.00	0.21^∗±0.20	0.25^∗b±0.00	0.02^∗b±0.00	0.37^∗b±0.00	0.00

4^# After challenge (at the 4th week).
∗Significant compared to control normal group in the same row.
bSignificant compared to control-infected group at the 4th week.
DHV: duck hepatitis virus.
LSD: least significant difference.