International Scholarly Research Notices / 2013 / Article / Tab 1

Research Article

On the Combined Application of Iatroscan TLC-FID and GC-FID to Identify Total, Neutral, and Polar Lipids and Their Fatty Acids Extracted from Foods

Table 1

Retention time, calibration data, and reproducibility for neutral and polar lipid standards.

Solvent systemLipid standardsRetention timeRegression equationCorrelation coefficients ( )CV% of intraday variabilityCV% of interday variability

Solvent A
(n-H : DE : FA) (60 : 15 : 1.5 by vol.)
HC0.02–0.03 0.99941.281.69
Sterol esters
(wax esters)
0.04–0.06 11.341.76
TG0.07–0.10 0.99842.331.83
FFA0.11–0.12 0.99261.572.21
1,3-DG0.16–0.18 0.99871.622.49
Sterol0.20–0.24 12.283.46
1,2-DG0.31–0.34 11.291.87
MG0.36–0.39 0.99931.562.17
PL0.42–0.45

Solvent B
(C : M : W)
(50 : 20 : 2, by vol.)
NL0.02–0.08
PE0.09–0.11 0.99871.742.86
PI0.11–0.14 0.99972.643.35
PS0.15–0.18 0.99972.473.68
l-PE0.19–0.20 0.99951.923.04
PnL0.21–0.23
PC0.24–0.30 0.99971.402.48
Sphm0.33–0.35 0.99941.762.55
l-PC0.37–0.40 0.99981.632.26

Development distance/times of development: 10 cm (1 time).

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