Table of Contents
ISRN Biotechnology
Volume 2013, Article ID 859830, 6 pages
Research Article

Potential of Aqueous Ozone to Control Aflatoxigenic Fungi in Brazil Nuts

1Institute for Biotechnology and Bioengineering IBB, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
2EMBRAPA Food Technology, Avenida das Américas 29501, 23020-470 Rio de Janeiro, RJ, Brazil

Received 31 May 2013; Accepted 26 June 2013

Academic Editors: S. Pan and M. Shoda

Copyright © 2013 Otniel Freitas-Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study aimed to verify the use of aqueous ozone as alternative technology for fungal control. Brazil nuts sterilized were inoculated with either or  conidia mL−1 of Aspergillus flavus (MUM 9201) to determine optimal treatment parameters and different aqueous ozone contact times. These assays showed that the effect of ozone is almost immediate against A. flavus, and the optimum ozone concentration depended on the number of initial viable spores on the shell. The remaining viable spores in the ozone solution were recorded, and the rate of inactivation for each treatment was determined by assessing the ratio between the cfu of each treatment and the control. The ozonized nuts were also cultured to recover the fungal population. Aqueous ozone was effective in reducing the conidia of A. flavus and the natural fungal population associated with Brazil nuts. Aqueous ozone presented a great potential to reduce microorganisms counts in Brazil nuts with a great potential use in packing houses for decontamination step.