Table of Contents
ISRN Veterinary Science
Volume 2013, Article ID 865702, 4 pages
Research Article

Use of a Multiplex PCR for the Detection of Toxin-Encoding Genes netB and tpeL in Strains of Clostridium perfringens

Department of Poultry Science, Auburn University, 201 Poultry Science Building, Auburn, AL 36849-5416, USA

Received 8 October 2013; Accepted 13 November 2013

Academic Editors: A. Balkema-Buschmann and A. Fernandez

Copyright © 2013 Matthew A. Bailey et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Some studies have shown that the NetB toxin may be an important virulence factor of Clostridium perfringens associated necrotic enteritis in poultry. Additionally, research has shown that strains of C. perfringens positive for both the netB gene and a second toxin-encoding gene, tpeL, appear to be more virulent than strains with only netB. In the past, detection of these genes has been performed relatively inefficiently using two single locus PCRs. This report describes a novel multiplex PCR developed to detect netB and tpeL simultaneously in C. perfringens strains isolated from cases of necrotic enteritis in broilers, providing a more efficient diagnostic tool in the screening of strains for these genes.