Research Article
Generation of Constitutive Active ERK Mutants as Tools for Cancer Research in Zebrafish
Table 1
Primer list for site-directed mutagenesis.
| | | Description | Sequence (5′ > 3′) | Reference |
| | MAPK1/ERK2 | ERK2/L84P_fw | CTGCGTGAGATTAAAATCCCGCTCCGCTTCAAGC | RefSeq: NM_182888 | | ERK2/L84P_rv | GCTTGAAGCGGAGCGGGATTTTAATCTCACGCAG | | ERK2/S162D_fw | CGAGACCTGAAGCCAGACAACCTGTTGCTCAACAC | | ERK2/S162D_rv | GTGTTGAGCAACAGGTTGTCTGGCTTCAGGTCTCG | | ERK2/D330N_fw | CCTGGAGCAGTACTATGATCCTACAAATGAGCCTGTTGCTGAG | | ERK2/D330N_rv | CTCAGCAACAGGCTCATTTGTAGGATCATAGTACTGCTCCAGG | | MAPK3/ERK1 | ERK1/L106P_fw | CTGAGAGAGATCAAAATCCCCCTGCGGTTCCATC | RefSeq: NM_201507 | | ERK1/L106P_rv | GATGGAACCGCAGGGGGATTTTGATCTCTCTCAG | | ERK1/S184D_fw | CAGAGACCTGAAGCCCGACAACCTTCTCATCAACAC | | ERK1/S184D_rv | GTGTTGATGAGAAGGTTGTCGGGCTTCAGGTCTCTG | | ERK1/D352N_fw | GCAGTACTATGACCCATCTAATGAGCCGGTAGCTGAGGAAC | | ERK1/D352N_rv | GTTCCTCAGCTACCGGCTCATTAGATGGGTCATAGTACTGC |
|
|
