Generation of Constitutive Active ERK Mutants as Tools for Cancer Research in Zebrafish

Table 3

Cluster 2 Peptides with increased phosphorylation specifically by FGF8 samples. Protein samples were prepared at 5 hpf. ANOVA and Dunnett’s test are performed with a biological triplicate.

Peptide	Fold change (mean 2log values)				𝑃 values versus WT			𝑃 values versus ERK2_WT
	WT	ERK2WT	ERK2***	FGF8	ERK2WT	ERK2***	FGF8	ERK2***	FGF8
ACM5_494_506	1.00	0.99	1.02	1.04	0.909	0.925	0.093	0.835	0.079
ACM5_498_510	1.00	0.98	1.01	1.08	0.897	0.730	0.119	0.637	0.143
ANDR_785_797	1.00	0.98	1.01	1.05	0.923	0.471	0.153	0.529	0.133
CA2D1_494_506	1.00	1.05	1.06	1.10	0.290	0.364	0.012^†	0.864	0.056
ESR1_160_172	1.00	0.97	0.99	1.04	0.451	0.189	0.183	0.523	0.060
GPR6_349_361	1.00	0.96	1.01	1.02	0.382	0.465	0.609	0.876	0.189
GSUB_61_73	1.00	0.98	0.93	1.05	0.441	0.147	0.942	0.432	0.402
KS6A1_374_386	1.00	1.00	1.02	1.09	0.969	0.822	0.430	0.852	0.409
P53_12_24	1.00	1.07	0.97	1.58	0.906	0.543	0.472	0.472	0.543

^†𝑃 values < 0.05.