Lesion-Induced Alterations in Astrocyte Glutamate Transporter Expression and Function in the Hippocampus

Figure 2

Lesion-induced reactive gliosis in organotypic slice cultures. (a) Schematic overview of the hippocampus indicating the position of the scratch wound. The box delineates the area primarily analyzed. (b) GFAP immunofluorescence and DAPI staining of the CA1 area in a control (left) and a lesioned (right) slice. The boxes indicate the areas enlarged below. The dashed line in the lower right indicates the lesion. (c) Double staining for GFAP (top) and S100β (bottom) adjacent to the lesion site. Note the gradual increase in GFAP and S100β expression towards the lesion. (b): widefield microscopy; (c): confocal microscopy (optical sections taken at 1 μm intervals). StrO, stratum oriens; StrP, stratum pyramidale; StrR, stratum radiatum; and StrLM, stratum lacunosum moleculare. Scale bars (b) 200 μm (top) and 50 μm (bottom); (c) 10 μm.