Lesion-Induced Alterations in Astrocyte Glutamate Transporter Expression and Function in the Hippocampus
Figure 7
D-aspartate-induced astrocyte sodium transients following lesion. (a) CA1 area of a control (left) and a lesioned (right) slice preparation. Shown are epifluorescence images of SR101 and SBFI fluorescences. Circled areas and numbers indicate the regions of interest analyzed in the experiment depicted in (b). StrP, stratum pyramidale; StrR, stratum radiatum; and scale bar: 40 μm. (b) Sodium transients induced by pressure application of 1 mM D-aspartate (D-asp) for 500 ms in the different cells in the control and lesioned slice as indicated in (a). (c) Histograms showing mean values ± S. E. M. of the peak amplitude (left), slope (center), and decay time (right) of D-aspartate-induced sodium transients in astrocytes in control slices () as well as in cells in the scar region and its periphery ( and , resp.). **; ***. StrP, stratum pyramidale; StrR, stratum radiatum; and scale bar: 40 μm.
