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International Scholarly Research Notices

International Scholarly Research Notices / 2013 / Article / Tab 1

Research Article

Lesion-Induced Alterations in Astrocyte Glutamate Transporter Expression and Function in the Hippocampus

Table 1

List of antibodies used.
AntibodyStructure of the immunogenCompanyOrder numberSpeciesTypeDilution
GLAST
(EAAT1)		KPYQLIAQDNEPEKPV
ADSETKM		Chemicon/MilliporeAB1782*Guinea
Pig IgG		Polyclonal1 : 1000
GLT-1
(EAAT2)		AANGKSADCSVEEEPW
KREK		Chemicon/MilliporeAB1783Guinea
Pig IgG		Polyclonal1 : 1000/1 : 2000
GFAPn.s. (full-length protein from cow spinal cord)Dako CytomationZ 0334Rabbit
IgG		Polyclonal1 : 1000/1 : 2000
GFAPclone G-A-5
(LQSLTCDVESLRGTNE
SLERQMREQEERHARE
AASYQEALTRLEEEGQ
SLKDEMARHLQEYQEL
LNVKLALDIEIATY)		Sigma-AldrichG3893Mouse
IgG		Monoclonal1 : 1000/1 : 2000
S100ßn.s. (recombinant full-length cow protein)Abcamab868Rabbit
IgG		Polyclonal1 : 100
Mouse
IgG		AF488 conjugatedInvitrogen (Life Technologies)A11029Goat—1 : 100
Rabbit
IgG		AF594 conjugatedInvitrogen (Life Technologies)A11012Goat—1 : 100
Guinea
pig IgG		AF488 conjugatedInvitrogen (Life Technologies)A11073Goat—1 : 100
GLAST: glutamate aspartate transporter; GLT-1: glutamate transporter 1; GFAP: glial fibrillary protein; AF: AlexaFluor; appl.: application; IHC: immunohistochemistry; WB: western blot; n.s.: not specified.
*The manufacturer has lately stopped the sale and distribution of the GLAST antibody used here.

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