Lesion-Induced Alterations in Astrocyte Glutamate Transporter Expression and Function in the Hippocampus

Table 1

List of antibodies used.

Antibody	Structure of the immunogen	Company	Order number	Species	Type	Dilution
GLAST; (EAAT1)	KPYQLIAQDNEPEKPV; ADSETKM	Chemicon/Millipore	AB1782*	Guinea; Pig IgG	Polyclonal	1 : 1000
GLT-1; (EAAT2)	AANGKSADCSVEEEPW; KREK	Chemicon/Millipore	AB1783	Guinea; Pig IgG	Polyclonal	1 : 1000/1 : 2000
GFAP	n.s. (full-length protein from cow spinal cord)	Dako Cytomation	Z 0334	Rabbit; IgG	Polyclonal	1 : 1000/1 : 2000
GFAP	clone G-A-5; (LQSLTCDVESLRGTNE; SLERQMREQEERHARE; AASYQEALTRLEEEGQ; SLKDEMARHLQEYQEL; LNVKLALDIEIATY)	Sigma-Aldrich	G3893	Mouse; IgG	Monoclonal	1 : 1000/1 : 2000
S100ß	n.s. (recombinant full-length cow protein)	Abcam	ab868	Rabbit; IgG	Polyclonal	1 : 100
Mouse; IgG	AF488 conjugated	Invitrogen (Life Technologies)	A11029	Goat	—	1 : 100
Rabbit; IgG	AF594 conjugated	Invitrogen (Life Technologies)	A11012	Goat	—	1 : 100
Guinea; pig IgG	AF488 conjugated	Invitrogen (Life Technologies)	A11073	Goat	—	1 : 100

GLAST: glutamate aspartate transporter; GLT-1: glutamate transporter 1; GFAP: glial fibrillary protein; AF: AlexaFluor; appl.: application; IHC: immunohistochemistry; WB: western blot; n.s.: not specified.
*The manufacturer has lately stopped the sale and distribution of the GLAST antibody used here.