A New Analytical Q-Absorbance Ratio Method Development and Validation for Simultaneous Estimation of Lamivudine and Isoniazid

Table 1

Calibration points of standard curve with standard deviation (SD) and %RSD.

Concentration of the solution (𝜇g/mL)	At 246 nm				At 272 nm
	LAM		INH		LAM		INH
	Mean absorbance ± SD (𝑛=3)	%RSD	Mean absorbance ± SD (𝑛=3)	%RSD	Mean absorbance ± SD (𝑛=3)	%RSD	Mean absorbance ± SD (𝑛=3)	%RSD
5	0.152±0.0015	1.007159	0.149±0.0020	1.400224	0.222±0.0025	1.132	0.144±0.0026	1.83733
10	0.288±0.0026	0.918664	0.287±0.0025	0.875851	0.435±0.0026	0.608	0.281±0.0049	1.75756
15	0.429±0.0040	0.941332	0.419±0.0040	0.965315	0.645±0.0035	0.544	0.409±0.0040	0.98894
20	0.569±0.0035	0.617565	0.538±0.0036	0.670177	0.836±0.007	0.837	0.525±0.0045	0.85945
25	0.715±0.0046	0.64092	0.687±0.0057	0.828093	1.038±0.0060	0.581	0.661±0.0065	0.98383
30	0.834±0.0055	0.660116	0.802±0.0060	0.751273	1.214±0.0095	0.786	0.813±0.0035	0.43214