A New Analytical Q-Absorbance Ratio Method Development and Validation for Simultaneous Estimation of Lamivudine and Isoniazid

Table 4

Summary of regression characteristics and validation parameters.

Parameters	LAM		INH
	246 nm	272 nm	246 nm	272 nm
Beer’s law limit (𝜇g/mL)	5–30	5–30	5–30	5–30
Absorptivity	287	435	288	276
Regression equation (𝑦=𝑚𝑥+𝑐)
Slope (𝑚); Intercept (𝑐)	0.0279; 0.0079	0.0405; 0.0191	0.0267; 0.0118	0.0266; 0.0065
Correlation coefficient (𝑟^2)	0.9994	0.9989	0.999	0.999
Standard deviation (SD)	0.0009	0.0023	0.0006	0.0017
LOD (𝜇g/mL)	0.106	0.186	0.078	0.211
LOQ (𝜇g/mL)	0.321	0.563	0.238	0.639
Sandell’s sensitivity (𝜇g/cm^2)	0.0347	0.0229	0.0348	0.0356