Table of Contents
ISRN Biotechnology
Volume 2013, Article ID 914187, 13 pages
Review Article

Challenges in Enzymatic Route of Mannitol Production

1Biotechnology Department, Lovely Professional University, Punjab 144 401, India
2Institute of Technology, Banaras Hindu University, Varanasi 221 005, India

Received 19 June 2012; Accepted 10 August 2012

Academic Editors: L. Betancor, H. S. Garcia, and J. Jia

Copyright © 2013 Sheelendra Mangal Bhatt et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.