Challenges in Enzymatic Route of Mannitol Production

Table 3

Km values of MDH for different substrates (source from BRENDA database).

Km value [mm]	Km value [mm] maximum	Substrate	Organism	Commentary	Literature
1.8	6.5	D-Arabinitol	Rhodobacter sphaeroides	—	[41]
0.44	—	D-Fructose	Pseudomonas fluorescens	in 50 mm glycine/NaOH buffer at pH 10	[55]
16.3	79.2	D-Fructose	Rhodobacter sphaeroides	—	[41]
50.97	—	D-Fructose	Thermotoga maritima	at 80°C and pH 6.1; at 80°C, pH 6.1	[46]
71	—	D-Fructose	Leuconostoc mesenteroides	pH 5.3	[52]
32	—	D-Glucitol	Rhodobacter sphaeroides	—	[41]
0.29	21.8	D-Mannitol	Platymonas subcordiformis	—	[56]
0.29	21.8	D-Mannitol	Rhodobacter sphaeroides	—	[41]
0.29	21.8	D-Mannitol	Saccharomyces cerevisiae	—	[57]
1.2	—	D-Mannitol	Pseudomonas fluorescens	recombinant protein	[58]
5.51	—	D-Mannitol	Thermotoga maritima	at 80°C and pH 6.1;	[41]
12	—	D-Mannitol	Leuconostoc pseudomesenteroides	pH 8.6, 30°C	[59]
13.23	—	D-Mannitol	Thermotoga maritima	at 60°C and pH 6.1	[41]
32	—	D-Mannitol	Leuconostoc mesenteroides	pH 8.6	[52]
78	—	D-Mannitol	Lactobacillus sanfranciscensis	25°C	[16]