Molecular Characterization of Begomoviruses and DNA Satellites Associated with a New Host Spanish Flag (Lantana camara) in India
Figure 1
(a) Agarose gel electrophoresis of the PCR products (lanes 1–10) of coat protein gene amplified (550 bp) from DNA extracts prepared from infected Lantana camara plants confirming positive results of begomovirus, whereas lane 11 stands for healthy plant, L represents the 100 bp ladder. (b) The presence of begomovirus was confirmed by Southern blot hybridization using Papaya leaf curl virus as a general probe for begomoviruses. Begomovirus from symptomatic plants hybridized with the probe, whereas samples extracted from healthy plants did not show positive results.