Research Article

Molecular Characterization of Begomoviruses and DNA Satellites Associated with a New Host Spanish Flag (Lantana camara) in India

Figure 1

(a) Agarose gel electrophoresis of the PCR products (lanes 1–10) of coat protein gene amplified ( 550 bp) from DNA extracts prepared from infected Lantana camara plants confirming positive results of begomovirus, whereas lane 11 stands for healthy plant, L represents the 100 bp ladder. (b) The presence of begomovirus was confirmed by Southern blot hybridization using Papaya leaf curl virus as a general probe for begomoviruses. Begomovirus from symptomatic plants hybridized with the probe, whereas samples extracted from healthy plants did not show positive results.
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(a)
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(b)