Multiple Roles for the sRNA GcvB in the Regulation of Slp Levels in Escherichia coli
Figure 2
slp and ompT mRNA levels from WT (GS162), ΔgcvB (GS1144), complemented (GS1144 [pGS594]), and GS1149 (ΔgcvBΔhfq) strains. The levels of slp and ompT mRNAs were determined by semiquantitative RT-PCR. Each reaction had 0.5 μg of total cellular RNA added for the RT reaction with 20 PCR cycles. The amplification product for slp was predicted to be 515 bps, and the amplification product for ompT was predicted to be 830 bps. When reverse transcriptase was omitted from the reactions, there was no product, indicating that no DNA was present in the RNA samples (data not shown).
