Multiple Roles for the sRNA GcvB in the Regulation of Slp Levels in Escherichia coli
Figure 3
β-Galactosidase levels of λ slp::lacZ lysogens. (a) WT (GS162), ΔgcvB (GS1144), Δhfq (GS1148), and ΔgcvBΔhfq (GS1149) λ slp::lacZ lysogens and the same lysogens transformed with the indicated plasmids were grown in LB (+ AP for complemented strains) to midlog phase of growth (OD600 ~ 0.5) and assayed for β-galactosidase activity. (b) WT (GS162), ΔgcvB (GS1144), Δhfq (GS1148), and ΔgcvΔhfq (GS1149) λ slp::lacZ lysogens or the same lysogens transformed with the indicated plasmids were grown in GM + glycine (+ AP for complemented strains) to midlog phase of growth (OD600 ~ 0.5) and assayed for β-galactosidase activity. Results are averages of two independent assays with each assay performed in triplicate.
