Multiple Roles for the sRNA GcvB in the Regulation of Slp Levels in Escherichia coli

Table 1

Strains and plasmids.

Strain or plasmid	Relevant genotype	Source or reference
Strain*
GS162	WT	This laboratory
GS1132	GS162 ΔgcvAΔgcvB	[10]
GS1144	GS162 ΔgcvB::ΩCM^r	[11]
GS1148	GS162 hfq-1::ΩCM^r	[12]
GS1149	GS162 Δ(gcvAgcvB)::ΩaadA hfq-1::ΩCM^r	[12]
Plasmid
pGS554	Single-copy vector + WT gcvB with a constitutive promoter (p𝑔𝑐𝑣𝐵^2+)	[10]
pGS571	Multicopy vector + WT gcvB (p𝑔𝑐𝑣𝐵^3+)	[13]
pGS594	Single-copy vector + WT gcvB (p𝑔𝑐𝑣𝐵^+)	This laboratory
pGS609	Multicopy vector + WT hfq (pℎ𝑓𝑞^3+)	[14]
pGS631	pGS594 with a change in gcvB at bp +131 and +132 of TT to CC (pgcvB + 131CC)	[15]
pGS632	pGS594 with a change in gcvB at bp +142 and +143 of TG to CA (pgcvB + 142CA)	[15]
pGS633	pGS594 with a change in gcvB at bp +159 and +160 of TG to CC (pgcvB + 159CC)	[15]
pGS634	pGS594 with a change in gcvB at bp +142 and +143 of TG to CA and bp +159 and +160 of TG to CC (pgcvB + 142CA + 159CC)	[15]

 *All strains also carry the pheA905 thi araD129 rpsL150 relA1 deoC1 flbB5301 ptsF25 rbsR mutations.