Multiple Roles for the sRNA GcvB in the Regulation of Slp Levels in Escherichia coli

Table 2

GcvB alters sensitivity to CM.*

Relevant genotype	Disk potency (30 μg CM); Zone diameter (mm)	𝑃 value compared to the WT strain	GT (min) grown in:
			LB	LB + CM
WT	22.19 ± 0.92		57 ± 3	198 ± 23
ΔgcvAΔgcvB	23.31 ± 0.88	0.0107	58 ± 2	237 ± 30
WT[p𝑔𝑐𝑣𝐵^3+]	21.06 ± 0.56	0.0260	58 ± 2	170 ± 5

 *WT (GS162), ΔgcvAΔgcvB (GS1132), and WT transformed with p𝑔𝑐𝑣𝐵³⁺ were grown overnight at 30°C (AP was added for the plasmid-carrying strain) and tested for CM sensitivity on Mueller-Hinton plates (see Materials and Methods). In addition, 50 μL of each overnight culture was used to inoculate 5 mL of LB with or without 3 μg mL⁻¹ CM, the cultures grown at 30°C and generation times (GT) measured. Average inhibition zones and standard deviations from eight Mueller-Hinton plates and average generation times and standard deviations of 3 independent growth experiments are reported.