Table of Contents
ISRN Developmental Biology
Volume 2013 (2013), Article ID 920265, 11 pages
http://dx.doi.org/10.1155/2013/920265
Research Article

Changes in -Tubulin Protein Distribution in Zebrafish (Danio rerio) Oocytes and the Early Cleavage-Stage Embryo

1Department of Biology, The University of Memphis, Memphis, TN 38152, USA
2Department of Physiology, University of Tennessee-Memphis, Memphis, TN 38163, USA
3Department of Biological Sciences, The University of Memphis, 223 Life Science Building, Memphis, TN 38152-3560, USA

Received 1 October 2012; Accepted 23 October 2012

Academic Editors: O. Bossinger and M. Lardelli

Copyright © 2013 Jianxiong Liu and Charles A. Lessman. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated the distribution of -tubulin in zebrafish oocytes and embryos using epifluorescent or confocal microscopy and -tubulin antibodies. During meiotic maturation of zebrafish oocytes, -tubulin begins redistribution from oocyte ooplasm and cortex to the future blastodisc region at the animal pole. In activated eggs, -tubulin was uniformly distributed in the enlarging blastodisc with label emanating from the yolk cell. In newly fertilized eggs, -tubulin was evenly distributed in blastomere cytoplasm, with the presence of pronuclei but initially lacking discernable centrosomes. During early cleavage, especially at the eight-cell stage, striking arc-shaped/rings (A/R) of putative centrosomes were detected. Decreasing -tubulin was seen in yolk cells while early cleavage blastomeres had strong cytoplasmic label along with obvious A/R arrays. In addition, we found the orientation of the A/R array and nuclear division alternated by about 90 degrees for each cell cycle along with alternation of punctate and A/R arrays.