Table of Contents
ISRN Hepatology
Volume 2013, Article ID 928485, 7 pages
http://dx.doi.org/10.1155/2013/928485
Research Article

Overexpression of Regulatory T Cells Type 1 (Tr1) Specific Markers in a Patient with HCV-Induced Hepatocellular Carcinoma

1CNRS UMR 8161, Institut de Biologie de Lille, 1 rue du Professeur Calmette, 59021 Lille Cedex, France
2UPMC Université Paris 6, Inserm, UMR-S938, Centre de Recherche Saint Antoine, 75012 Paris, France
3Service de Chirurgie Digestive et Transplantations, Hôpital Huriez, CHU, Université Nord-de-France, 59000 Lille, France
4Centre de Transplantation Hépatique, Pôle Digestif, APHP, Hôpital Saint Antoine, 184 rue de Faubourg Saint Antoine, 75012 Paris, France

Received 27 June 2013; Accepted 24 July 2013

Academic Editors: D. Capone, A. Castiella, and O. Topcu

Copyright © 2013 Laurissa Ouaguia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hepatitis C virus (HCV) is an important causative agent of liver disease, but factors that determine the resolution or progression of infection are poorly understood. In this study, we suggested that existence of immunosuppressive mechanisms, supported by regulatory T cells and especially the regulatory T cell 1 subset (Tr1), may explain the impaired immune response during infection and thus the fibrosis aggravation to hepatocellular carcinoma (HCC). Using quantitative real-time PCR, we investigated the intra-hepatic presence of Tr1 cells in biopsies from a genotype 1b infected patient followed for an 18-year period from cirrhosis to HCC. We described a significant increase of gene expression in particular for the cytokines IL-10, TGF-β, and their receptors that were perfectly correlated with an increased expression of the Tr1 specific markers (combined expression of CD4, CD18, and CD49b). This was strongly marked since the patient evolved in the pathology and could explain the failure of the treatment. In conclusion, evidence of regulatory T cell installation in the liver of chronically infected patient with cirrhosis and HCC suggests for the first time a key role for these cells in the course of HCV infection.