Effect of Extruder Screw Speed, Temperature, and Enzyme Levels on Sugar Recovery from Different Biomasses

Table 4

Effect of screw speed and temperature on sugar recovery from big bluestem.

Screw speed, rpm	Temperature, ^°C
	100	150	200	100	150	200	100	150	200
	1 cellulase : 1 β-glucosidase			1 cellulase : 4 β-glucosidase			1 multienzyme : 1 β-glucosidase
Glucose
100	17.8^e	33.0^c	46.9^a	31.2^c	43.3^a-c	53.3^ab	17.5^a	17.4^a	18.2^a
150	25.7^d	34.6^c	42.6^b	41.7^a-c	45.3^a-c	47.2^a-c	17.6^a	17.7^a	17.3^a
200	33.9^c	43.2^b	48.2^a	37.1^bc	57.5^a	54.3^ab	17.1^a	18.6^a	18.5^a
Xylose
100	9.6^e	10.7^e	32.0^b	46.7^b	56.2^ab	60.9^ab	3.8^e	4.0^e	22.2^b
150	10.3^e	18.0^d	32.2^b	55.2^ab	57.8^ab	57.5^ab	5.4^de	8.9^d	19.4^bc
200	16.8^d	28.1^c	37.4^a	50.9^ab	66.2^a	63.3^a	6.8^de	16.3^c	26.8^a
Combined sugar
100	15.0^e	25.6^c	40.8^a	33.1^c	44.6^a-c	51.4^ab	13.3^b	13.2^b	18.9^a
150	20.4^d	13.8^e	37.8^b	43.4^a-c	45.7^a-c	46.5^a-c	13.6^b	14.7^ab	17.5^a
200	27.8^c	36.9^b	42.9^a	38.7^a-c	57.6^a	53.2^ab	13.9^b	17.5^a	20.4^a

Control: 20.3% glucose, 34.1% xylose, and 22.5% combined sugar (with 1 : 4 cellulase to β-glucosidase). Superscripts with same letter within column are not significantly different for each sugar under each enzyme combination.